The effects of internally and externally directed attention during motor skill execution and learning

Focusing attention onto the intended outcome or goal of a movement (an External focus of attention) has been shown to be more beneficial to the learning and performance of movements than focusing onto the components of the movement being carried out (an Internal focus of attention). In this thesis, four studies assessed the effects of attentional focusing strategies on the learning and execution of motor skills during different situations. Study 1 demonstrated that an internal focus of attention during a suprapostural pointing task resulted in degraded postural control as well as larger movements of the hand and arm. In Study 2 novices using an external focus were more accurate in a dart throwing task than those using an internal focus, but no different from a control condition. In Study 3 two experiments investigated the effects of attentional focuses on postural control at rest and whilst fatigued. Postural control was no better using external focus when compared to an internal focus at rest, but was better than baseline. When fatigued (localised and generalised), balance was significantly deteriorated using an external focus, but not when an internal focus was used. In two experiments during Study 4 novices carrying out a dart throwing task used different attentional focusing instructions during practice and later performance. During practice sessions in Experiment 4.1 and 4.2 accuracy was not affected by attentional focusing instructions. Using an external focus during performance resulted in significantly better accuracy than using an internal focus. In Experiment 4.2, novices who preferred an internal focus but used an external focus during performance performed less accurately than participants who preferred the external focus. Findings demonstrate that the benefits of an external focus of attention is evident in performance situations, whereas an internal focus may be beneficial whilst fatigued and is not detrimental during practice

The Groundwater Drought Initiative (GDI): analysing and understanding groundwater drought across Europe

Major droughts, such as recent events in 2011-12, 2015 and 2017-18, exhibit spatial coherence across Europe and have been the subject of spatio-temporal monitoring and analysis of the driving meteorology, surface and soil water droughts and vegetation status. However, to date there has been no such equivalent monitoring or analysis of groundwater droughts at the pan-European scale – this represents a significant gap in drought research and therefore in water management. Groundwater drought, defined as below normal groundwater levels, is a threat to water security across Europe. Groundwater resources are highly susceptible to major multi-seasonal to multi-annual episodes of meteorological drought due to the pooling, lagging and lengthening of drought signals in groundwater systems. These phenomena are dependent on river catchment and aquifer characteristics and may be impacted by anthropogenic influences such as abstraction and land use management, having potentially important consequences for society and ecosystems. To address this research gap, the Groundwater Drought Initiative (GDI), a pan-European collaboration, is undertaking a large-scale data synthesis of groundwater level data across Europe to produce the first assessment of spatio-temporal changes in groundwater drought status from ~1960 to the present, and the first systematic assessment of the impacts of groundwater drought at the European scale. Here we describe the methods used to undertake this continental scale status and impact assessment including illustrations from regional-scale analysis of drought in the Chalk aquifer of the UK. Facilitating and underpinning these activities, the GDI is establishing a new network to co-ordinate groundwater drought research across Europe. We welcome all contributions to the Initiative and explain how to become involved with the GDI

The Groundwater Drought Initiative (GDI): analysing and understanding groundwater drought across Europe

In Europe, it is estimated that around 65 % of drinking water is extracted from groundwater. Worryingly, groundwater drought events (defined as below normal groundwater levels) pose a threat to water security. Groundwater droughts are caused by seasonal to multi-seasonal or even multi-annual episodes of meteorological drought during which the drought propagates through the river catchment into the groundwater system by mechanisms of pooling, lagging, and lengthening of the drought signals. Recent European drought events in 2010–2012, 2015 and 2017–2018 exhibited spatial coherence across large areas, thus demonstrating the need for transboundary monitoring and analysis of groundwater level fluctuations. However, such monitoring and analysis of groundwater drought at a pan-European scale is currently lacking, and so represents a gap in drought research as well as in water management capability. To address this gap, the European Groundwater Drought Initiative (GDI), a pan-European collaboration, is undertaking a large-scale data synthesis of European groundwater level data. This is being facilitated by the establishment of a new network to co-ordinate groundwater drought research across Europe. This research will deliver the first assessment of spatio-temporal changes in groundwater drought status from ∼1960 to present, and a series of case studies on groundwater drought impacts in selected temperate and semi-arid environments across Europe. Here, we describe the methods used to undertake the continental-scale status assessment, which are more widely applicable to transboundary or large-scale groundwater level analyses also in regions beyond Europe, thereby enhancing groundwater management decisions and securing water supply

When Pinocchio's nose does not grow:belief regarding lie-detectability modulates production of deception

Does the brain activity underlying the production of deception differ depending on whether or not one believes their deception can be detected? To address this question, we had participants commit a mock theft in a laboratory setting, and then interrogated them while they underwent functional MRI (fMRI) scanning. Crucially, during some parts of the interrogation participants believed a lie detector was activated, whereas in other parts they were told it was switched off. We were thus able to examine the neural activity associated with the contrast between producing true versus false claims, as well as the independent contrast between believing that deception could and could not be detected. We found increased activation in the right amygdala and inferior frontal gyrus (IFG), as well as the left posterior cingulate cortex (PCC), during the production of false (compared to true) claims. Importantly, there was a significant interaction between the effects of deception and belief in the left temporal pole and right hippocampus/parahippocampal gyrus, where activity increased during the production of deception when participants believed their false claims could be detected, but not when they believed the lie detector was switched off. As these regions are associated with binding socially complex perceptual input and memory retrieval, we conclude that producing deceptive behavior in a context in which one believes this deception can be detected is associated with a cognitively taxing effort to reconcile contradictions between one’s actions and recollections

What are the priority research questions for digital agriculture?

There is a need to identify key existing and emerging issues relevant to digitalisation in agricultural production that would benefit from a stronger evidence base and help steer policy formulation. To address this, a prioritisation exercise was undertaken to identify priority research questions concerning digital agriculture in the UK, but with a view to also informing international contexts. The prioritisation exercise uses an established and effective participatory methodology for capturing and ordering a wide range of views. The method involves identifying a large number of participants and eliciting an initial long list of research questions which is reduced and refined in subsequent voting stages to select the top priorities by theme. Participants were selected using purposive sampling and snowballing to represent a number of sectors, organisations, companies and disciplines across the UK. They were each invited to submit up to 10 questions according to certain criteria, and this resulted in 195 questions from a range of 40 participants (largely from England with some representation from Scotland and Wales). Preliminary analysis and clustering of these questions through iterative analysis identified seven themes as follows: data governance; data management; enabling use of data and technologies; understanding benefits and uptake of data and technologies; optimising data and technologies for performance; impacts of digital agriculture; and new collaborative arrangements. Subsequent stages of voting, using an online ranking exercise and a participant workshop for in-depth discussion, refined the questions to a total of 27 priority research questions categorised into 15 gold, 7 silver and 5 bronze, across the 7 themes. The questions significantly enrich and extend previous clustering and agenda setting using literature sources, and provide a range of new perspectives. The analysis highlights the interconnectedness of themes and questions, and proposes two nexus for future research: the different dimensions of value, and the social and institutional arrangements to support digitalisation in agriculture. These emphasise the importance of interdisciplinarity and transdisciplinarity, and the need to tackle the binary nature of current analytical frames. These new insights are equally relevant to contexts outside the UK. This paper highlights the need for research actions to inform policy, not only instrumentally by strengthening the evidence base, but also conceptually, to prompt new thinking. To our knowledge this methodology has not been previously applied to this topic

Active site specificity profiling of the matrix metalloproteinase family: Proteomic identification of 4300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses

Secreted and membrane tethered matrix metalloproteinases (MMPs) are key homeostatic proteases regulating the extracellular signaling and structural matrix environment of cells and tissues. For drug targeting of proteases, selectivity for individual molecules is highly desired and can be met by high yield active site specificity profiling. Using the high throughput Proteomic Identification of protease Cleavage Sites (PICS) method to simultaneously profile both the prime and non-prime sides of the cleavage sites of nine human MMPs, we identified more than 4300 cleavages from P6 to P6′ in biologically diverse human peptide libraries. MMP specificity and kinetic efficiency were mainly guided by aliphatic and aromatic residues in P1′ (with a ~ 32–93% preference for leucine depending on the MMP), and basic and small residues in P2′ and P3′, respectively. A wide differential preference for the hallmark P3 proline was found between MMPs ranging from 15 to 46%, yet when combined in the same peptide with the universally preferred P1′ leucine, an unexpected negative cooperativity emerged. This was not observed in previous studies, probably due to the paucity of approaches that profile both the prime and non-prime sides together, and the masking of subsite cooperativity effects by global heat maps and iceLogos. These caveats make it critical to check for these biologically highly important effects by fixing all 20 amino acids one-by-one in the respective subsites and thorough assessing of the inferred specificity logo changes. Indeed an analysis of bona fide MEROPS physiological substrate cleavage data revealed that of the 37 natural substrates with either a P3-Pro or a P1′-Leu only 5 shared both features, confirming the PICS data. Upon probing with several new quenched-fluorescent peptides, rationally designed on our specificity data, the negative cooperativity was explained by reduced non-prime side flexibility constraining accommodation of the rigidifying P3 proline with leucine locked in S1′. Similar negative cooperativity between P3 proline and the novel preference for asparagine in P1 cements our conclusion that non-prime side flexibility greatly impacts MMP binding affinity and cleavage efficiency. Thus, unexpected sequence cooperativity consequences were revealed by PICS that uniquely encompasses both the non-prime and prime sides flanking the proteomic-pinpointed scissile bond

The First Post-Kepler Brightness Dips of KIC 8462852

We present a photometric detection of the first brightness dips of the unique variable star KIC 8462852 since the end of the Kepler space mission in 2013 May. Our regular photometric surveillance started in October 2015, and a sequence of dipping began in 2017 May continuing on through the end of 2017, when the star was no longer visible from Earth. We distinguish four main 1-2.5% dips, named "Elsie," "Celeste," "Skara Brae," and "Angkor", which persist on timescales from several days to weeks. Our main results so far are: (i) there are no apparent changes of the stellar spectrum or polarization during the dips; (ii) the multiband photometry of the dips shows differential reddening favoring non-grey extinction. Therefore, our data are inconsistent with dip models that invoke optically thick material, but rather they are in-line with predictions for an occulter consisting primarily of ordinary dust, where much of the material must be optically thin with a size scale <<1um, and may also be consistent with models invoking variations intrinsic to the stellar photosphere. Notably, our data do not place constraints on the color of the longer-term "secular" dimming, which may be caused by independent processes, or probe different regimes of a single process

Large-Scale Gene-Centric Meta-Analysis across 39 Studies Identifies Type 2 Diabetes Loci

To identify genetic factors contributing to type 2 diabetes (T2D), we performed large-scale meta-analyses by using a custom similar to 50,000 SNP genotyping array (the ITMAT-Broad-CARe array) with similar to 2000 candidate genes in 39 multiethnic population-based studies, case-control studies, and clinical trials totaling 17,418 cases and 70,298 controls. First, meta-analysis of 25 studies comprising 14,073 cases and 57,489 controls of European descent confirmed eight established T2D loci at genome-wide significance. In silico follow-up analysis of putative association signals found in independent genome-wide association studies (including 8,130 cases and 38,987 controls) performed by the DIAGRAM consortium identified a T2D locus at genome-wide significance (GATAD2A/CILP2/PBX4; p = 5.7 x 10(-9)) and two loci exceeding study-wide significance (SREBF1, and TH/INS; p <2.4 x 10(-6)). Second, meta-analyses of 1,986 cases and 7,695 controls from eight African-American studies identified study-wide-significant (p = 2.4 x 10(-7)) variants in HMGA2 and replicated variants in TCF7L2 (p = 5.1 x 10(-15)). Third, conditional analysis revealed multiple known and novel independent signals within five T2D-associated genes in samples of European ancestry and within HMGA2 in African-American samples. Fourth, a multiethnic meta-analysis of all 39 studies identified T2D-associated variants in BCL2 (p = 2.1 x 10(-8)). Finally, a composite genetic score of SNPs from new and established T2D signals was significantly associated with increased risk of diabetes in African-American, Hispanic, and Asian populations. In summary, large-scale meta-analysis involving a dense gene-centric approach has uncovered additional loci and variants that contribute to T2D risk and suggests substantial overlap of T2D association signals across multiple ethnic groups

Loci influencing blood pressure identified using a cardiovascular gene-centric array

Blood pressure (BP) is a heritable determinant of risk for cardiovascular disease (CVD). To investigate genetic associations with systolic BP (SBP), diastolic BP (DBP), mean arterial pressure (MAP) and pulse pressure (PP), we genotyped 50 000 single-nucleotide polymorphisms (SNPs) that capture variation in 2100 candidate genes for cardiovascular phenotypes in 61 619 individuals of European ancestry from cohort studies in the USA and Europe. We identified novel associations between rs347591 and SBP (chromosome 3p25.3, in an intron of HRH1) and between rs2169137 and DBP (chromosome1q32.1 in an intron of MDM4) and between rs2014408 and SBP (chromosome 11p15 in an intron of SOX6), previously reported to be associated with MAP. We also confirmed 10 previously known loci associated with SBP, DBP, MAP or PP (ADRB1, ATP2B1, SH2B3/ATXN2, CSK, CYP17A1, FURIN, HFE, LSP1, MTHFR, SOX6) at array-wide significance (P 2.4 10(6)). We then replicated these associations in an independent set of 65 886 individuals of European ancestry. The findings from expression QTL (eQTL) analysis showed associations of SNPs in the MDM4 region with MDM4 expression. We did not find any evidence of association of the two novel SNPs in MDM4 and HRH1 with sequelae of high BP including coronary artery disease (CAD), left ventricular hypertrophy (LVH) or stroke. In summary, we identified two novel loci associated with BP and confirmed multiple previously reported associations. Our findings extend our understanding of genes involved in BP regulation, some of which may eventually provide new targets for therapeutic intervention.</p